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Organic Chemistry Seminar
April 9, 2019 @ 12:30 pm - 1:30 pm
Abigail Bravo (Kang Group)
Enzymatic EDTA Degradation Pathway
Ethylenediaminetetraacetic acid (EDTA) is a water-soluble chelating agent commonly used in vast range of applications. Its recalcitrance to degradation, and potential to mobilize radionuclides and other heavy metals has caused EDTA to become a major organic pollutant of concern in U.S surface water. Two bacterial species from the genus Chelativorans have been identified as having the ability to use EDTA as a sole source of carbon and nitrogen. EDTA is transported through the periplasm by EppA, a periplasmic substrate-binding protein, and across the periplasmic membrane by the ABC-transporter (EppB, C, and D). Previous studies have characterized the first enzymes in the degradation pathway, a two-component flavin-diffusible monooxygenase system comprised of EDTA monooxygenase (EmoA), and an NADH:FMN oxidoreductase(EmoB), and interaction between the two. My research has focused on characterization of the following step, oxidation of EDDA and IDA by the enzyme iminodiacetate oxidase (IdaA), and the transcriptional regulator, EmoR, of the EDTA degradation pathway.